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Figure |
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Extended Life-Span
Conferred by Cotransporter Gene Mutations
in Drosophila |
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Volume 18,
Issue 1, 2002 |
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Life-span extension in
Indy mutants. Survival curves of males heterozygous for three
different Indy mutations, a precise excision of the P -element from
Indy 302 (revertant), and an enhancer-trap control are shown. All flies
were tested as heterozygotes over a wild-type Canton-S strain. The Indy
mutants are Indy302 (open green circles), Indy206 (solid
blue circles), and Indy159 (open orange circles). The excision line
(open red squares) is one of four exact excisions (sequence confirmed) of
the P element obtained by mobilizing the P element from either the
Indy302 or Indy206 line, using delta 2-3 transposase. The
control (solid black squares) is one of four other enhancertrap control
lines from the same mutagenesis that generated Indy302 and
Indy206, tested as a heterozygote over Canton-S. A similar control
survival curve was found for a control from the mutagenesis that gave rise
to Indy159. The mean 25°C life-spans of controls were 37 days,
whereas the mean life-spans for Indy206, Indy302, and
Indy159 were 71, 69, and 69 days, respectively. Indy206,
Indy302, and Indy159 extended mean life-span by 92, 87, and 87%
respectively. Extension of 1% maximal life-span of these Indy
mutants was greater than 45%. At 18°C, the increase in mean
life-span conferred by Indy mutations approached 100%,
whereas the increase in 1% maximum life-span approached 50%. Flies were
maintained in a humidified, temperature-controlled environmental chamber
at 25°C and were transferred to fresh food vials and scored for
survival every 2 to 3 days as in. Each survivorship curve represents data
from over 300 male flies. A total of 5430 male and female Indy
heterozygote flies were tested. |
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 Rogina
B, et al. Science 290:2137-2140, 2000. |
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